Are you looking to grow or practice growing crystals? Try out some of these crystallization experiments.

Industry Standard Tetragonal Lysozyme Recipe Lysozyme: 50 mg/ml in 0.1 M Sodium Acetate pH 4.8 Reagent: 8% w/v Sodium Chloride, 0.1 M Sodium Acetate pH 4.8 Additional Reagents: Index Reagent 8, 22, 28, 31, 34, 40, 58, 59, 69, 86, 88 Mix equal amounts of lysozyme with reagent, incubate at 4 or 22 degrees Celsius. Batch or vapor diffusion works fine.

The 15 Minute Lysozyme Crystals - By Enrico Stura Lysozyme: 100 mg/ml in 50 mM Sodium Acetate pH 4.5 Reagent: 30% w/v MPEG 5,000, 1.0 M Sodium Chloride, 50 mM Sodium Acetate pH 4.5 Mix equal amount of lysozyme with reagent. Dilute protein and/or MPEG and/or Sodium Chloride for less nucleation, larger, and better shaped crystals. Batch or vapor diffusion method works fine.

A Sweet Thaumatin Recipe Thaumatin: 25 to 50 mg/ml in your choice of buffer (ADA, ACES, Bis-Tris) at about pH 6.5 Reagent: 1.5 M Potassium Sodium Tartrate or 1.5 M Sodium Tartrate Additional Reagents: Index Reagent 26, 31, 78, 86 Mix equal amounts of Thaumatin with reagent and equilibrate vapor diffusion style over reagent.

Hampton's Glucose Isomerase Glucose Isomerase: 20-30 mg/ml in water or 50 mM buffer pH 6 to 8 Reagent: Your choice or the following (1.5-2.5 M Ammonium Sulfate pH 6-9) or (10-15% w/v PEG 4000-8000, 0.2 M salt*, pH 6-9 0.6), or (1.0 M Sodium Citrate, pH 6-8), or (0.1-0.3 M Magnesium Formate), or (1.0 M Sodium Formate, pH 5-7), or (10-20% v/v MPD, 0.2 M salt*, pH 6-9), or (10-20% v/v PEG 400, 0.2 M salt*, pH 6-9), or 10-20% iso-Propanol, 0.2 M salt*, pH 6-9) * = 0.2 M salt such as Ammonium Sulfate, Magnesium Chloride, Magnesium Acetate, Calcium Acetate, or Ammonium Acetate. Mix equal amounts of glucose isomerase and reagent and equilibrate vapor diffusion style over reagent.

Alpha Lactalbumin from Cow Milk - By Alexander McP Alpha Lactalbumin: 30 to 40 mg/ml in water or 10 mM Tris-HCl buffer pH 8.5 Reagent: 30% w/v PEG 3350, 0.10 M Tris HCl pH 8.5, 0.2 M Lithium Sulfate Mix equal amounts of Alpha Lactalbumin and reagent and equilibrate vapor diffusion style over reagent.

Classic Catalase Beef Liver Catalase: 30 mg/ml in 25 mM Hepes pH 7 Reagent: Crystal Screen 25, 36 Mix equal amounts of Catalase with reagent. Vapor diffusion works great.

Precious Ribonuclease S Bovine Pancrease Ribonuclease S: 50 mg/ml in 25 mM Hepes pH 7.5 Reagent: Crystal Screen 9, 20 Mix equal amounts of Ribonuclease S with reagent. Vapor diffusion method.

Pounds of Proteinase K Proteinase K: 20 mg/ml in 25 mM Hepes pH 7.0, PMSF Reagent: Crystal Screen 2, 3, 4, 6, 7, 9, 10, 11, 15, 16, 17, 18, 20, 22, 25, 28, 29, 30, 31, 32, 33, 34, 35, 36, 38, 39, 40, 41, 42, 44, 45, 46, 47, 48, 49, 50 Mix equal amounts of Proteinase K with reagent. Vapor diffusion or microbatch works well. This stuff grows anytime, anywhere, in anything... almost.

Crystals for Cryo Glucose Isomerase: 20 to 30 mg/ml in 25 mM Tris pH 7.5 Reagent: Crystal Screen Cryo Reagent 21 Mix equal amounts of Glucose Isomerase and reagent. Vapor diffusion method. Mount crystal in CryoLoop. Swish mounted crystal through reservoir to increase MPD concentration in crystal to decrease mosaicity. No swish = more mosaicity. Crystal can diffract to 1.6, indexes in I-centered orthorhombic with a=92.7 b=97.4 c=102.7. Mosaicity is about 0.5 t0 0.6 Reagent: Crystal Screen Cryo Reagent 23 Mix equal amounts of Glucose Isomerase and reagent. Vapor diffusion method. Mount crystal in CryoLoop. Mosaicity may be a bit more in this reagent and the unit cell will shrink a tad. Special thanks to Laurie Betts for the the cryo work and data!

Tricky Trypsin Bovine Pancreas Trypsin: 60 mg/ml in 10 mM Calcium Chloride, 10 mg/ml Benzamidine Hydrochloride, 25 mM Hepes pH 7.0 Reagent: Crystal Screen 4, 15, 16, 20, 28, 30, 31 Mix equal amounts of Trypsin with reagent. Vapor diffusion method works nicely.