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MakeTray is a free, web based program which generates both a pipetting worksheet and a plate formulation document for crystallization set ups. MakeTray allows one to enter general information about the sample and experiment which is printed on the pipetting worksheet and the formulation document. The plate size can be customized for any number of wells, so MakeTray works for 24, 48 and 96 well plates. The reservoir volume is adjustable but must be the same for each well. A reservoir can include up to 12 unique chemicals or additions. MakeTray is especially useful for optimization experiments.
Instructions for using MakeTray
General
Information
Information such as Experiment ID, Sample Name, Your Name and
Sample Concentration is entered under the section entitled "General
Information". This information will be printed on the pipetting worksheet and
formulation sheet.
Setting Up The Plate Size
Enter Tray Number.
Unique tray numbers can be used to further identify the experiment. Enter the
number of reservoirs in the X direction (typically printed 1, 2, 3, 4 ... on the
plate) and the Y direction (typically printed A, B, C, D ... on the plate). A 24
well plate would be created by entering 6 for X and 4 for Y. A 96 well plate
would be created by entering 12 for X and 8 for Y. To simply generate a set of
dilutions or pipetting instructions for a single reservoir, enter
1.
Reagent Number 1-12
Between 1 to 12 additions can be
accommodated with MakeTray. Enter the reagent name and the stock concentration
and select the concentration units. For each reagent, select the desired
dilution option. The dilution option can be the same or different for each of
the reagents.
Set of concentrations in the "X" direction: List the
desired final reservoir concentration, separating each entry with a space. For
example, enter 1 1.2 1.4 1.6 1.8 2.0 to have this be the final concentration of
a reagent across the 6 columns in a VDX Plate.
Set of concentrations
in the "Y" direction: List the desired final reservoir concentration,
separating each entry with a space. For example, enter 1 1.2 1.4 1.6 to have
this be the final concentration of a reagent down the 4 rows in a VDX
Plate.
Increase in the "X" direction from the lowest value of "?" in
steps of "?": Enter 1 in the first field and 0.3 in the second field to have
the reagent concentration be 1 in column 1, 1.3 in column 2, 1.6 in column 3,
1.9 in column 4, 2.2 in column 5 and 2.5 in column 6.
"Increase in the
"Y" direction from the lowest value of "?" in steps of "?": Enter 10 in the
first field and 5 in the second field to have the reagent concentration be 10 in
row 1, 15 in row 2, 20 in row 3 and 25 in row 4.
Constant
concentration of "?" throughout the entire tray: Enter 0.2 to have a
concentration of 0.2 in each and every reservoir of the tray.
Constant
concentration of "?" in column number "?" (starting from the left): Enter
0.5 in the first field and 4 in the second field to have the first three columns
of the plate contain none of the reagent in question, and then starting with
column 4, have a concentration of 0.5.
Constant concentration of "?"
in row number "?" (starting from the top): Enter 0.01 in the first field and
3 in the second field to have the first two rows of the plate contain none of
the reagent in question, and then starting with row 3, have a concentration of
0.01.
Build Tray
Click "Clear fields" to clear all the fields
for the desired reagent number. Click "Build Tray" to have MakeTray create the
pipetting worksheet and formulation sheet. "Clear fields" and "Build Tray" are
located at the end of each "Reagent Number" area for convenience.
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